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Incremento da produção de metabolitos secundários através de técnicas de cultura in vitro: caraterização química e genética de plantas comestíveis, suas propriedades bioativas e microencapsulação das frações fenólicas | Improving secondary metabolites production through in vitro culture technique: chemical and genetic characterization of edible plants, bioactive properties and microencapsulation of phenolic fractions

Breve Descrição | Brief Description:
In vitro culture is a worldwide used methodology for the production of secondary metabolites. Herein, it will be applied to four wild plant species from Trás-os-Montes: Achillea millefolium (mil-folhas), Taraxacum officinale (dente de leão), Fragaria vesca (morango silvestre) and Laurus nobilis (loureiro). The aim is to establish and optimize in vitro culture to stimulate the production of secondary metabolites with bioactive properties. To achieve this, different parameters related to stress induction will be controlled, and the obtained in vitro cultured plants differentiated by comparing their genetic profile with both wild and cultivated samples. After chemical characterization of in vitro cultured, cultivated and wild samples, the antioxidant and antitumor activity of their phenolic fractions will be evaluated. The most bioactive fractions and its isolated compounds will be microencapsulated in order to preserve biological, chemical and functional properties and achieve an effective controlled release for successful pharmaceutical and/or food applications.

Equipa | Team:
Isabel C.F.R. Ferreira (Coordenadora- CIMO-IPB)
Rita C. Alves (REQUIMTE)
Maria Filomena Barreiro (LRSE-IPB)
Maria Inês Dias

Instituições | Institutions:
1) Centro de Investigação de Montanha, ESA, Instituto Politécnico de Bragança, Campus Santa Apolónia Ap. 1172, 5301-855 Bragança, Portugal.
2) REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia da Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
3) Laboratório de Processos de Separação e Reacção (LSRE), Laboratório associado LSRE/LCM, Instituto Politécnico de Bragança, Campus Santa Apolónia Ap. 1134, 5301-857 Bragança, Portugal.

Calendário | Dates:
2013-2017

Financiamento | Financial support:
1) Fundação para a Ciência e a Tecnologia (FCT, Portugal) pela Bolsa Individual de doutoramento de Maria Inês Dias (SFRH/BD/84485/2012).
2) Centro de Investigação de Montanha (PEst-OE/AGR/UI0690/2011)
3) REQIMTE (PEst-C/EQB/LA0006/2011)
4) LRSE (PEst-C/EQB/LA0020/2011)

Sítio do projecto | Project website:
www.esa.ipb.pt/biochemcore

Contacto | contact:
Isabel C.F.R. Ferreira – iferreira@ipb.pt; ESA, Instituto Politécnico de Bragança, Campus Santa Apolónia Ap. 1172, 5301-855 Bragança, Portugal

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